| 货号 | 3993S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | MAVS Antibody detects endogenous levels of total human MAVS protein. The bands detected at 52 and 75 kDa correlate with those described by Seth et al. (2005). |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | The mitochondrial antiviral signaling protein (MAVS, VISA) contributes to innate immunity by triggering IRF-3 and NF-κB activation in response to viral infection, leading to the production of IFN-β (1). The MAVS protein contains an N-terminal CARD domain and a C-terminal mitochondrial transmembrane domain. The MAVS adaptor protein plays a critical and specific role in viral defenses (2). MAVS acts downstream of the RIG-I RNA helicase and viral RNA sensor, leading to the recruitment of IKKε, TRIF and TRAF6 (3,4). Some viruses have evolved strategies to circumvent these innate defenses by using proteases that cleave MAVS to prevent its mitochondrial localization (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 75, 52 |
Western blot analysis of extracts from LNCaP, MCF-7, and HT29 cell lines using MAVS Antibody.Western免疫印迹。用MAVS Antibody研究LNCaP, MCF-7, 和HT29 细胞系的细胞提取液。 | |
Western blot analysis of extracts from HeLa cells, mock-transfected or transfected with human MAVS, using MAVS Antibody.Western免疫印迹。用MAVS Antibody研究未转染和转染了人MAVS的HeLa细胞的细胞提取液。 | |
Confocal immunofluorescent analysis of MCF-7 cells using MAVS Antibody (green) showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).共聚焦免疫荧光分析MCF-7细胞，所用抗体为 MAVS Antibody(绿色)图片展示了用MitoTracker® Red CMXRos (红色)标记的线粒体。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。 |
