| 货号 | 4542S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-F/IF-IC |
| 目标/特异性 | MAP2 Antibody detects endogenous levels of all isoforms of MAP2 total protein. This antibody does not cross-react with tau. |
| 使用方法 | WB(1:1000) IF-F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. Serine 136, threonine 1620 and threonine 1623 of MAP2 are phosphorylated by GSK3 and p44/42 MAP kinases (2,3). Phosphorylation at threonine 1620/1623 by GSK3 inhibits MAP2 association with microtubules and microtubule stability (3). |
| 存放说明 | -20C |
| 计算分子量 | 75, 82, 280 |
Western blot analysis of extracts from COS cells, untransfected or MAP2C transfected, using MAP2 Antibody. (Human MAP2C construct is a generous gift from Dr. B. Shafit-Zagardo, Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY.) Western blot分析未转染或转染MAP2C的COS细胞，使用的抗体是MAP2 Antibody 兔多抗。（人HAP2C载体由Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY的Dr. B. Shafit-Zagardo赠送） | |
Western blot analysis of extracts from PC12 cells, untreated or nocodazole-treated, using MAP2 Antibody. Western blot分析未处理或nocodazole处理的PC12细胞，使用的抗体是MAP2 Antibody 兔多抗。 | |
Confocal microscopic images of PC-12 cells showing cytoplasmic stain with MAP2 Antibody (A, red) compared to an isotype control (B). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). PC-12细胞的共聚焦显微图像显示出MAP2 Antibody 兔多抗对胞质染色（A图，红色），与同型对照比对（B图）。蓝色伪彩=DRAQ5® #4084(荧光DNA染料)。 | |
Confocal immunofluorescent analysis of rat cerebellum, hippocampus and spinal cord (as indicated) using MAP2 Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析大鼠小脑、海马和脊髓，使用的抗体是MAP2 Antibody 兔多抗（绿色）。蓝色伪彩=DRAQ5® #4084 (荧光DNA染料)。 |
