| 货号 | 12399S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Malic Enzyme (Mitochondrial) Antibody recognizes endogenous levels of total malic enzyme (mitochondrial) protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Cytosolic malic enzyme (ME1) catalyzes the conversion of malate and NADP+ to pyruvate and NADPH (1,2). NADPH is then used for fatty acid biosynthesis and lipogenesis (1,2). Cytosolic malic enzyme was shown to mediate high fat diet-induced adiposity (1). Mitochondrial malic enzyme (ME2) preferentially uses NAD+ to catalyze the conversion of malate to pyruvate with the concomitant generation of NADH (2). Recent studies have demonstrated that the tumor suppressor p53 regulates cell metabolism and proliferation by repressing the expression of both cytosolic malic enzyme and mitochondrial malic enzyme (3). |
| 存放说明 | -20C |
| 计算分子量 | 65 |
Western blot analysis of extracts from various cell lines using Malic Enzyme (Mitochondrial) Antibody. Western blot 检测多种细胞系提取物，使用抗体为 Malic Enzyme (Mitochondrial) Antibody。 | |
Immunoprecipitation of malic enzyme (mitochondrial) from 293 cell extracts, using Normal Rabbit IgG #2729 (lane 2) or Malic Enzyme (Mitochondrial) Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Malic Enzyme (Mitochondrial) Antibody. 免疫沉淀方法检测293细胞提取物苹果酸酶（线粒体）蛋白，使用抗体为Normal Rabbit IgG #2729 (泳道2)或Malic Enzyme (Mitochondrial) Antibody (泳道3)。泳道1为10%对照。使用Malic Enzyme (Mitochondrial) Antibody进行Western blot分析。 | |
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with either a construct expressing DDK-tagged full-length human malic enzyme (cytoplasmic) (hME1-DDK; +) or a construct expressing DDK-tagged full-length human malic enzyme (mitochondrial) (hME2-DDK; +), using Malic Enzyme (Mitochondrial) Antibody (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower). Western blot方法检测293细胞提取物：空质粒转染（-）或带DDK-标签全长苹果酸酶（胞质）(hME1-DDK; +)及带DDK-标签全长苹果酸酶（线粒体）(hME2-DDK; +)载体转染；使用抗体为Malic Enzyme (Mitochondrial) Antibody（上图），DYKDDDDK Tag (9A3) Mouse mAb鼠单抗 #8146（中图），或β-Actin (D6A8) Rabbit mAb兔单抗 #8457（下图）。 |
