| 货号 | 5195S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Lipin 1 Antibody detects endogenous levels of total Lipin 1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Lipin 1 was identified as a nuclear protein required for adipose tissue development (1). The expression of Lipin 1 is induced during adipocyte differentiation (1). The abnormal development of adipose tissues caused by mutations in the lipin 1 gene results in lipodystrophy, a condition associated with low body fat, fatty liver, hypertriglyceridemia, and insulin resistance (1). Lipin 1 plays a role in lipid metabolism in various tissues and cell types including liver, muscle, adipose tissues, and neuronal cell lines (2-4). It has dual functions at the molecular level: Lipin 1 serves as a transcriptional coactivator in liver, and a phosphatidate phosphatase in triglyceride and phospholipid biosynthesis pathways (5). Lipin1 is regulated by mTOR, illustrating a connection between adipocyte development and nutrient-sensing pathways (6). It also mediates hepatic insulin signaling by TORC2/CRTC2 (7). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
Confocal immunofluorescent analysis of 3T3-L1 adipocytes using Lipin 1 Antibody (red). Lipid droplets were labeled with BODIPY® 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光检测3T3-L1 细胞，所用抗体为Lipin 1 Antibody（红），脂质滴用BODIPY® 493/503 标记（绿）。蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5® #4084) | |
Western blot analysis of extracts from human white pre-adipocytes and human white adipocytes using Lipin 1 Antibody (upper) and β-Actin Antibody #4967 (lower). Wetern blot检测人白色脂肪前脂肪细胞提取物和人白色脂肪细胞提取物，所用抗体为Lipin 1 Antibody (上)和β-Actin Antibody #4967 (下)。 |
