| 货号 | 3842S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | LIMK1 Antibody detects endogenous levels of total LIMK1 protein independent of phosphorylation. The antibody does not cross-react with LIMK2. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing the LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Western blot analysis of extracts from HEK293, A431, NIH/3T3, COS and PC12 cells, using LIMK1 Antibody. 使用LIMK1 Antibody，免疫印迹（Western Blot）分析HEK293、A431、NIH/3T3、COS 和PC12细胞LIMK1蛋白水平。 |
