| 货号 | 2621S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | JMJD1B/JHDM2B Antibody detects endogenous levels of JMJD1B protein (all three isoforms). |
| 使用方法 | WB(1:1000) IP (1:25) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain of several proteins has been shown to catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into seven separate families (3). The JMJD1 (Jumonji domain-containing protein 1) family, also known as JHDM2 (JmjC domain-containing histone demethylation protein 2) family, contains four members: hairless (HR), JMJD1A/JHDM2A, JMJD1B/JHDM2B, and JMJD1C/JHDM2C. Hairless is expressed in the skin and brain and acts as a co-repressor of the thyroid hormone receptor (4-6). Mutations in the hairless gene cause alopecia in both mice and humans (4,5). JMJD1A is expressed in meiotic and post-meiotic male germ cells, contributes to androgen receptor-mediated gene regulation, and is required for spermatogenesis (7-9). It has also been identified as a downstream target of OCT4 and STAT3 and is critical for the regulation of self-renewal in embryonic stem cells (10,11). JMJD1B is a more widely expressed family member and is frequently deleted in myeloid leukemia (12). JMJD1C (also known as TRIP8) is a co-factor of both the androgen and thyroid receptors and has a potential link to autism (13-15). Members of the JMJD1/JHDM2 family have been shown to demethylate mono-methyl and di-methyl histone H3 (Lys9) (3,8). |
| 存放说明 | -20C |
| 计算分子量 | 220 |
Confocal immunofluorescent analysis of HeLa cells using JMJD1B/JHDM2B Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). 使用JMJD1B/JHDM2B Antibody (绿色)标记，共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of whole cell lysates from HeLa, RAW and COS cells using JMJD1B/JHDM2B Antibody. 使用JMJD1B/JHDM2B Antibody，免疫印迹(Western blot)分析HeLa、RAW和COS细胞中JMJD1B/JHDM2B的蛋白水平。 |
