| 货号 | 9244S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | IκB-ζ Antibody detects endogenous levels of total human IκB-ζ protein and transfected levels of mouse IκB-ζ. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). |
| 存放说明 | -20C |
| 计算分子量 | 85 |
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with mouse IκB-ζ, using IκB-ζ Antibody.Western免疫印迹。用IκB-ζ Antibody研究模拟转染和转染了鼠源IκB-ζ的HeLa细胞的细胞提取液。 | |
Western blot analysis of extracts from THP-1 cells, untreated (-) or treated with TPA #4174 (200 nM, 48 hr; +) or LPS (1 μg/ml, 24 hr, +), using IκB-ζ Antibody. |
