| 货号 | 5681S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | IWS1 Antibody detects endogenous levels of total IWS1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Various steps in gene expression, such as mRNA processing, surveillance, export, and synthesis are coupled to transcription elongation (1,2). The C-terminal domain (CTD) of the large subunit of RNA polymerase II plays an important role in the integration of these different steps (1,2). IWS1 interacts with Spt6, a CTD-binding transcription elongation factor and H3 chaperone (1,2). IWS1 also recruits another CTD-binding protein, HYPB/Setd2 histone methyltransferase, to the RNA polymerase II complex for elongation-coupled H3K36 trimethylation (2). Thus, IWS1 links Spt6 and HYPB/Setd2 in a large complex and regulates mRNA synthesis and histone methylation at the co-transcriptional level (2).基因表达的不同阶段如 mRNA 加工、监控、输出和合成都与转录延伸相偶联(1,2)。RNA聚合酶II一个大亚基的C末端结构域 (CTD)在这些不同步骤的整合中起到了重要的作用 (1,2)。IWS1蛋白与一个CTD结合转录延伸因子及H3伴侣的Spt6蛋白相互作用(1,2)。IWS1也招募另外一个CTD结合蛋白HYPB/Setd2组蛋白甲基转移酶到RNA聚合酶II复合物上，以使延伸偶联的H3K36三甲基化 (2)。因此，IWS1在一个大的复合物中与Spt6和HYPB/Setd2相互联系，并且在共转录水平上调节mRNA合成和在组蛋白甲基化(2)。 |
| 存放说明 | -20C |
| 计算分子量 | 140 |
Western blot analysis of extracts from various cell lines using IWS1 Antibody. 使用IWS1 Antibody，免疫印迹（Western blot）分析不同细胞系的提取物。 | |
Confocal immunofluorescent analysis of NIH/3T3 cells using IWS1 Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). 使用IWS1 Antibody（绿色）标记，共聚焦免疫荧光观察IWS1蛋白在NIH/3T3细胞定位，分为紫外未照射组和紫外照射组。DY-554 phalloidin标记微丝蛋白（红色）。 |
