| 货号 | 4964S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 目标/特异性 | IRF-4 Antibody detects endogenous levels of IRF-4 protein. The antibody does not cross-react with other family members at physiological levels. |
| 使用方法 | WB(1:1000) IP (1:50) ChIP (1:25) |
| 供应商 | CST |
| 背景 | Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2). |
| 存放说明 | -20C |
| 计算分子量 | 51 |
Western blot analysis of extracts from Ramos, Raji, and HuT-78 cells, using IRF-4 Antibody.Western免疫印迹。用IRF-4 Antibody研究Ramos, Raji和 HuT-78细胞的细胞提取液。 | |
Western blot analysis of extracts from Karpas-620 (human) and A20 (mouse) cell lines using IRF-4 Antibody #4964 and IRF-4 (P173) Antibody #4948.Western免疫印迹。用IRF-4 Antibody #4964 和 IRF-4 (P173) Antibody #4948研究KARPAS-620 (人源)和A20 (小鼠源)细胞系的细胞提取液。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 H929 cells and either 20 μl of IRF-4 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human SUB1 Promoter Primers #5156 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。 H929细胞培养至4 x 106，用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用20 μl IRF-4 Antibody或2 μl Normal Rabbit IgG #2729 。用SimpleChIP® Human SUB1 Promoter Primers #5156 和SimpleChIP® Human α Satellite Repeat Primers #4486对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。 |
