| 货号 | 3808S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | IRAP Antibody detects endogenous levels of total IRAP protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | IRAP (also known as LNPEP) was originally described as an insulin-responsive aminopeptidase found in Glut4-containing vesicles (1). It is essentially always in the same compartments as Glut4 and has identical insulin-stimulated translocation patterns as Glut4 (2). IRAP is therefore considered to be a surrogate marker for Glut4 (2). IRAP was later found to be a critical enzyme that regulates the expression and activity of several essential hormones and regulatory proteins, including the Glut4 transporter (3,4). This membrane associated, zinc-dependent cystinyl aminopeptidase acts as both a receptor for angiotensin IV as well as the enzyme that catalyzes the synthesis of this essential hormone from its angiotensinogen precursor (5). IRAP catalyzes the hydrolysis of several peptide hormones, including oxytocin and vasopressin (4). Abnormal IRAP expression or activity is associated with several forms of cancer in humans, including renal and endometrial cancers (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 165 |
Western blot analysis of extracts from C2C12 cells using IRAP Antibody. Wetern blot检测C2C12细胞提取物，所用抗体为IRAP Antibody。 | |
Confocal immunofluorescent analysis of differentiated 3T3-L1 cells using IRAP Antibody (red). Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光检测分化的3T3-L1细胞，所用抗体为IRAP Antibody（红），脂肪滴用BODIPY 493/503 (green)染色。蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5 #4084)。 |
