| 货号 | 4367S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | IRAK2 Antibody detects endogeneous levels of total IRAK2 protein. Cross-reactivity was not detected with other family members. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88 and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm and activates protein kinase cascades, which include TAK1, IKKs and the stress-activated kinases (3). |
| 存放说明 | -20C |
| 计算分子量 | 62 |
Western blot analysis of extracts from Jurkat, Raji and K562 cell lines, using IRAK2 Antibody #4367.Western免疫印迹。用 IRAK2 Antibody #4367研究 Jurkat, Raji 和 K562细胞的细胞提取液。 | |
Immunofluorescent staining of HeLa cells, using IRAK2 Antibody.免疫荧光染色HeLa细胞，所用抗体为IRAK2 Antibody。 |
