| 货号 | 4359S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | IRAK1 (Human Specific) Antibody detects endogenous levels of total IRAK1 protein. No cross reactivity was detected with other family members at physiological conditions. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88 and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm and activates protein kinase cascades, which include TAK1, IKKs and the stress-activated kinases (3). |
| 存放说明 | -20C |
| 计算分子量 | 78-105 |
Western blot analysis of extracts from HeLa, 293, and MCF7 cells using IRAK1 (Human Specific) Antibody.Western免疫印迹。用IRAK1 (Human Specific) Antibody研究HeLa, 293 和 MCF7细胞的细胞提取液。 | |
Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24h) and treated with 1 μg/ml LPS for the indicated times, using IRAK1 Antibody (Human Specific).Western免疫印迹。用IRAK1 Antibody (Human Specific)研究经 TPA (#9905, 80 nM，24h)分化后经1 μg/ml LPS处理的 THP-1细胞的细胞提取液。 | |
Confocal immunofluorescent analysis of HT-1080 cells using IRAK1 Antibody (Human Specific) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye ).共聚焦免疫荧光分析HT-1080DLD-1 细胞，所用抗体为IRAK1 Antibody (Human Specific)(绿色)。肌动蛋白微丝用 DY-554 phalloidin (红色)标记。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。 |
