| 货号 | 13945S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 使用方法 | WB(1:1000) IP (1:100) ChIP (1:50) |
| 供应商 | CST |
| 背景 | The integrator complex is an evolutionarily conserved complex that is composed of at least 12 subunits in humans. It is thought to be a multifunctional complex with roles in orchestrating snRNA 3 end processing with transcription termination, DNA double-stranded break repair, hematopoietic development, and cell cycle progression (1-6). The integrator subunits (INTS) 9 and 11 are thought to be the catalytic subunits of the complex and are essential for the function of the complex (6,7). Research studies indicate that the integrator complex is recruited to snRNA genes through its interaction with the carboxy-terminal domain (CTD) of Rpb1, the largest subunit of RNA polymerase II (8). Phosphorylation of the Rpb1 CTD heptapeptide repeat residues Ser2 and Ser7 is required for efficient binding of integrator subunit proteins (9). |
| 存放说明 | -20C |
| 计算分子量 | 75 |
Western blot analysis of extracts from various cell lines using INTS9 Antibody. | |
Immunoprecipitation of INTS9 from HCT 116 cell extracts using Normal Rabbit IgG #2729 (lane 2) or INTS9 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using INTS9 Antibody. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 µl of INTS9 Antibody or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human RNU2-1 Promoter Primers #14084 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
