Integrin α4 Antibody
货号：
4600S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 4600S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Integrin α4 Antibody detects endogenous levels of integrin α4 mature protein (150kDa), α4 precursor protein (140kDa), as well as 70 kDa cleaved C-terminal α4 fragment. |
| 使用方法 | WB(1:1000)
IP (1:100) |
性能| 供应商 | CST |
| 背景 | Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).
A pair of important α4 integrins, α4β1 and α4β7, interact with VCAM-1, fibronectin and MAdCAM-1 at cell adhesions (3). Gene knockout and antibody blocking research reveal that α4 integrins play important roles in embryonic liver and heart development and in fetal lymphocyte homing (4-6). Phosphorylation at Ser988 within the cytoplasmic tail of integrin α4 blocks integrin α4 binding to paxillin and promotes leading edge migration (7,8). Integrin α4 is potentially a key therapeutic target in the treatment of inflammatory bowel disease and multiple sclerosis (9,10).
On SDS PAGE, Integrin α4 can migrate at several different apparent molecular sizes, a 150 kDa mature protein, and a 140 kDa precursor protein (a 180 kDa protein also exists under mild non-reducing conditions) (11). α4 has a cleavage site at R558, which results in a small portion of the protein as either a 80 kDa N-terminal or a 70 kDa C-terminal fragment (12). |
| 存放说明 | -20C |
| 计算分子量 | 70, 140, 150, (180) |
参考图片Western blot analysis of extracts from MOLT-4 and Jurkat cells using Integrin α4 Antibody.使用整合素α4 抗体对MOLT-4和Jurkat细胞提取物进行western blot分析。 |
