| 货号 | 3862S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | ILK1 Antibody detects endogenous levels of total ILK1 protein. The antibody may cross-react with ILK2. |
| 使用方法 | WB(1:1000) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility (1). ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin (1). ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin (2-5). These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP (1,3,6). It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1 (2). |
| 存放说明 | -20C |
| 计算分子量 | 51 |
Western blot analysis of extracts from HeLa, NIH/3T3, COS, PC12 and C6 cells, using ILK1 Antibody.使用ILK1抗体HeLa, NIH/3T3, COS, PC12 和C6细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using ILK1 Antibody.使用ILK1抗体i石蜡包埋的人乳腺癌组织进行免疫组化分析，显示细胞质定位。 | |
Western blot analysis of extracts from HeLa cells transfected with 50 nM non-targeted siRNA (-) or 100 nM ILK1 siRNA (+), using ILK1 Antibody #3862 and Cofilin Antibody #3312. The ILK1 Antibody confirms silencing of ILK1 expression, and the Cofilin Antibody controls for protein loading and ILK1 siRNA specificity.转染50 nM未标记siRNA(-)或100 nM ILK1 siRNA(+)的HeLa细胞提取物，使用ILK1抗体#3862和Cofilin抗体#3312进行western blot分析。ILK1抗体用以确认ILK1的敲除，Cofilin抗体用作蛋白上杨和ILK1 siRNA特异性的对照。 |
