| 货号 | 2685S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | IKKγ Antibody detects endogenous levels of total IKKγ protein independent of phosphorylation. It does not cross-react with IKKα or IKKβ. |
| 使用方法 | WB(1:1000) IHC-P (1:50) |
| 供应商 | CST |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation; Ser177 and Ser181 in the activation loop of IKKβ (serine 176 and 180 in IKKα) are the specific sites whose phosphorylation causes conformational changes resulting in kinase activation (10-13). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
Western blot analysis of extracts from various cell lines using IKKγ Antibody.Western免疫印迹。用IKKγ Antibody研究各种细胞系的细胞提取液。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic and membrane localization, using IKKγ Antibody.免疫组织化学染色分析石蜡包埋人乳腺癌组织。图示为细胞质和细胞膜的定位，所用抗体为IKKγ Antibody。 |
