| 货号 | 3027S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | IGF-I Receptor beta Antibody detects endogenous levels of IGF-IR beta. It does not cross-react with insulin receptor. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:2400) |
| 供应商 | CST |
| 背景 | Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
Immunohistochemical analysis of paraffin-embedded human breast tumor section showing membrane and cytoplasmic staining, using IGF-I Receptor b Antibody. 对石蜡包埋的人乳腺肿瘤切片使用IGF-I Receptor b Antibody进行免疫组化分析，显示细胞膜和细胞质定位。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using IGF-I Receptor b Antibody. 对石蜡包埋的人前列腺癌使用IGF-I Receptor b Antibody进行免疫组化分析。 | |
Western blot analysis of various cell extracts using IGF-I Receptor beta antibody. 对多种细胞抽提液使用IGF-I Receptor beta Antibody抗体进行Western blot分析。 |
