| 货号 | 2773S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F |
| 目标/特异性 | Huntingtin Antibody detects endogenous levels of total huntingtin protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:100) |
| 供应商 | CST |
| 背景 | Huntingtin Disease (HD) is a fatal neurodegenerative disorder characterized by psychiatric, cognitive, and motor dysfunction. Neuropathology of HD involves specific neuronal subpopulations: GABA-ergic neurons of the striatum and neurons within the cerebral cortex selectively degenerate (1,2). The genetic analysis of HD has been the flagship study of inherited neurological diseases from initial chromosomal localization to identification of the gene. Huntingtin is a large (340-350 kD) cytosolic protein that may be involved in a number of cellular functions such as transcription, gastrulation, neurogenesis, neurotransmission, axonal transport, neural positioning, and apoptosis (2,3). The HD gene from unaffected individuals contains between 6 and 34 CAG trinucleotide repeats, with expansion beyond this range causing the onset of disease symptoms. A strong inverse correlation exists between the age of onset in patients and the number of huntingtin gene CAG repeats encoding a stretch of polyglutamine peptides (1,2). The huntingtin protein undergoes numerous post-translational modifications including phosphorylation, ubiquitination, sumoylation, palmitoylation, and cleavage (2). Phosphorylation of Ser421 by Akt can partially counteract the toxicity that results from the expanded polyglutamine tract. Varying Akt expression in the brain correlates with regional differences in huntingtin protein phosphorylation; this pattern inversely correlates with the regions that are most affected by degeneration in diseased brain (2). A key step in the disease is the proteolytic cleavage of huntingtin protein into amino-terminal fragments that contain expanded glutamine repeats and translocate into the nucleus. Caspase mediated cleavage of huntingtin at Asp513 is associated with increased polyglutamine aggregate formation and toxicity. Phosphorylation of Ser434 by CDK5 protects against cleavage (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 350 |
Confocal immunofluorescent analysis of adult rat brain using Huntingtin Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 共聚焦免疫荧光分析成年大鼠大脑细胞，使用的抗体是Huntingtin Antibody 兔多抗（绿色）。蓝色伪彩=DRAQ5™（荧光DNA染料)。 | |
Western blot analysis of extracts from rat and mouse brain using Huntingtin Antibody. Western blot分析大鼠和小鼠大脑提取物，使用的抗体是Huntingtin Antibody 兔多抗。 |
