| 货号 | 5380S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | hnRNP A1 (R196) Antibody detects both isoforms of endogenous hnRNP A1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). hnRNP A1 regulates the alternative splicing of c-Src and c-H-Ras (2,3) and modifies initiation of translation of the fibroblast growth factor 2 mRNA (4). hnRNP A1 expression level is elevated in many cancers; knockdown of hnRNP A1 leads to apoptosis in various cancer cells (5). Although predominantly nuclear, hnRNP A1 is continually transported from the nucleus to the cytoplasm where it disassociates from mRNA and is rapidly re-imported into the nucleus (6,7). hnRNP A1 binds to cis-acting repressive sequences (CRS) of HIV-1 to influence HIV-1 production (8,9). HIV-1 enhances hnRNP A1 expression and promotes the relocalization of hnRNP A1 to the cytoplasm (10). |
| 存放说明 | -20C |
| 计算分子量 | 34, 40 |
Confocal immunofluorescent analysis of HeLa cells using hnRNP A1 (R196) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用hnRNP A1 (R196) Antibody（绿色），共聚焦免疫荧光观察hnRNP A1蛋白在HeLa细胞定位。DY-554 phalloidin标记微丝蛋白（红色）。 | |
Western blot analysis of extracts from HeLa and 293T cells using hnRNP A1 (R196) Antibody. 使用hnRNP A1 (R196) Antibody，免疫印迹（Western Blotting）分析HeLa和293T细胞系的提取物。 |
