| 货号 | 5692S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | HMGN1 Antibody recognizes endogenous levels of total HMGN1 protein. This antibody does not cross-react with other HMGN proteins, including HMGN2, HMGN3, HMGN4, and HMGN5. |
| 使用方法 | WB(1:1000) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | High mobility group (HMG) proteins are a superfamily of abundant and ubiquitous nuclear proteins that bind DNA without sequence specificity and induce structural changes to the chromatin fiber to regulate access to the underlying DNA. The HMGN family of proteins, which includes five members (HMGN1-5), is characterized by the presence of several conserved protein domains: a positively charged domain, a nucleosome binding domain, and an acidic C-terminal chromatin-unfolding domain (1,2). HMGN proteins function in transcriptional regulation and are recruited to gene promoters by transcription factors such as estrogen receptor alpha (ERα), serum responsive factor (SRF) and PITX2, where they can facilitate either gene activation or repression (3-5). HMGN proteins bind specifically to nucleosomal DNA and reduce the compaction of the chromatin fiber, in part by competing with linker histone H1 for nucleosome binding (6). In addition, HMGN proteins act to modulate local levels of post-translational histone modifications, decreasing phosphorylation of histone H3 at Ser10 and histone H2A at Ser1, and increasing acetylation of histone H3 at Lys14 (7-9). HMGN proteins can also modulate the activity of several chromatin-remodeling factors and restrict nucleosome mobility (10). |
| 存放说明 | -20C |
| 计算分子量 | 18 |
Confocal immunofluorescent analysis of COS-7 cells using HMGN1 Antibody (green). Actin filaments were labeled with DY-554 phallodin (red). 使用HMGN1 Antibody (绿色)标记，共聚焦免疫荧光分析COS-7细胞。DY-554 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of extracts from various cell lines using HMGN1 Antibody. 使用HMGN1 Antibody，免疫印迹(Western blot)分析不同细胞中HMGN1的蛋白水平。 |
