| 货号 | 2718S |
| 反应种属 | Human/Mouse/Rat/Monkey/Zebrafish |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Histone H2A.Z Antibody detects endogenous levels of histone H2A.Z protein. The antibody does not cross-react with other histone proteins, including histone H2A. |
| 使用方法 | WB(1:1000) IP (1:25) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Modulation of chromatin structure plays a critical role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure (1). There are five major variants of histone H2A: canonical H2A (most abundant), H2A.X, MacroH2A, H2ABbd and H2A.Z (2). Histone H2A.Z, the most conserved variant across species, functions as both a positive and negative regulator of transcription and is important for chromosome stability (2). Several homologous protein complexes, such as SWR-C ( S. cerivisiae), TIP60 (D. melanogaster) and SRCAP (mammals), have been shown to catalyze the ATP-dependent exchange of H2A.Z for H2A in the nucleosome (3,4,5). This exchange of histone H2A variants changes histone-histone interactions in the nucleosome core and alters an acidic patch on the surface of the nucleosome, resulting in changes in nucleosome stability and binding of non-histone proteins such as HP1α (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 14 |
Confocal immunofluorescent analysis of HeLa cells, using Histone H2A.Z Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). 使用Histone H2A.Z Antibody (绿色)标记，共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of HeLa, NIH/3T3, H-4-II-E and COS cell lysates, in addition to 10 ng of recombinant H2A (rH2A) and H2A.Z (rH2A.Z) protein, using Histone H2A.Z Antibody #2718 (upper) and Histone H2A Antibody II #2578 (lower). 使用Histone H2A.Z Antibody #2718 (上图)和Histone H2A Antibody II #2578 (下图)，免疫印迹(Western blot)分析HeLa、NIH/3T3、H-4-II-E和COS细胞中Histone H2A.Z和Histone H2A的蛋白水平。 |
