| 货号 | 2578S |
| 反应种属 | Human/Mouse/Rat/Monkey/Zebrafish |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Histone H2A Antibody II detects endogenous levels of total histone H2A protein, but not cross-react with other histones. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) |
| 供应商 | CST |
| 背景 | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). |
| 存放说明 | -20C |
| 计算分子量 | 14 |
Western blot Analysis of extracts from NIH/3T3, HeLa and C6 cells using Histone H2A Antibody II. 使用Histone H2A Antibody II，免疫印迹(Western blot)分析NIH/3T3、HeLa和C6细胞系中Histone H2A的蛋白水平。 | |
Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with TSA, serum or Calyculin A as indicated using Histone H2A Antibody II (upper) or Acetyl-Histone H2A (Lys5) Antibody #2576 (lower). 使用Histone H2A Antibody II (上图)或Acetyl-Histone H2A (Lys5) Antibody #2576 (下图)，免疫印迹(Western blot)分析NIH/3T3细胞系中Histone H2A的蛋白水平，如图所示细胞分为未处理组或处理组： TSA、serum或Calyculin A。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear localization using Histone H2A Antibody II. 使用Histone H2A Antibody II，免疫组化分析人源肺癌组织石蜡切片，结果显示为细胞核定位。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Histone H2A Antibody II. 使用Histone H2A Antibody II，免疫组化分析人源乳腺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Histone H2A Antibody II. 使用Histone H2A Antibody II，免疫组化分析人源结肠癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Histone H2A Antibody II. 使用Histone H2A Antibody II，免疫组化分析人源前列腺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkins lymphoma using Histone H2A Antibody II. |
