| 货号 | 2545S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | HDAC2 Antibody (IP Preferred) detects endogenous levels of total HDAC2 protein and is the preferred antibody for immunoprecipitation. The antibody does not cross-react with other HDAC proteins. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Western blot analysis of extracts from 293 and DLD-1 cells using HDAC2 Antibody (IP Preferred). 使用HDAC2 Antibody (IP Preferred)，免疫印迹(Western blot)分析293和DLD-1细胞中HDAC2的蛋白水平。 | |
Immunoprecipitation/Western blot analysis of extracts from 293 cells. Lane 1 contains lysate input (5%), lane 2 was immunoprecipitated with HDAC2 Antibody (IP Preferred) and lane 3 was immunoprecipitated with non-specific rabbit IgG. Western blot analysis was performed using HDAC2 Antibody (IP Preferred). 免疫沉淀/免疫印迹分析293细胞。第1道包含裂解物input (5%)，第2道与HDAC2 Antibody(IP Preferred)免疫沉淀，第3道与非特异性rabbit IgG免疫沉淀。使用HDAC2 Antibody (IP Preferred)进行免疫印迹(Western blot)分析。 |
