| 货号 | 4906S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Hamartin/TSC1 Antibody detects endogenous levels of total hamartin/TSC1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Tuberous sclerosis complex (TSC) is an autosomal dominant disorder that causes symptoms including hamartomas in brain, kidney, heart, lung and skin (1). The tumor suppressor genes TSC1 and TSC2 encode hamartin and tuberin, respectively (2,3). Hamartin and tuberin form a functional complex and are involved in numerous cellular activities such as vesicular trafficking, regulation of the G1 phase of the cell cycle, steroid hormone regulation, Rho activation and anchoring neuronal intermediate filaments to the actin cytoskeleton (4-9). The combination of genetic, biochemical and cell-biological studies demonstrate that the tuberin/hamartin complex functions as a GTPase-activating protein for the Ras-related small G protein Rheb and thus inhibits targets of rapamycin including mTOR. Cells lacking hamartin or tuberin fail to inhibit phosphorylation of S6 kinase resulting in the activation of S6 ribosomal proteins translation of 5TOP mRNA transcripts (10). Hamartin is phosphorylated by CDK1 (cdc2) at Thr417, Ser584 and Thr1047 in cells in G2/M phase of the cell cycle (11). |
| 存放说明 | -20C |
| 计算分子量 | 150 to 170 |
Western blot analysis of extracts from NIH/3T3, MDA-MB-468 and PC12 cells, untreated or lambda phosphatase-treated, using Hamartin/TSC1 Antibody. Western blot分析NIH/3T3, MDA-MB-468 和 PC12细胞的提取物，未处理组和γ磷酸酶处理组，所用抗体为Hamartin/TSC1 Antibody。 |
