| 货号 | 9338S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | GSK-3alpha Antibody detects endogenous levels of total GSK-3α protein. It does not cross-react with recombinant GSK-3β. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). GSK-3alpha regulates the production of amyloid-beta peptides, a major component of the plaques that accumulate with progression of Alzheimers disease. Administration of therapeutic concentrations of lithium, a GSK-3 inhibitor, attenuates amyloid-beta production by specifically inhibiting the cleavage of amyloid precursor protein (APP) by gamma secretase, blocking accumulation of amyloid-beta peptides in the brains of mice that overproduce APP (6). |
| 存放说明 | -20C |
| 计算分子量 | 51 |
Western blot analysis of recombinant GST-GSK-3beta protein (76kD), and extracts from HeLa and PC3 cells, using GSK-3alpha Antibody (left) and GSK-3beta Antibody (right). Western blot 分析重组GST-GSK-3β 蛋白 (76kD)和HeLa 与 PC3 细胞提取物，所用抗体为GSK3a Antibody (左) 和GSK-3β Antibody (右). | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® GSK-3α siRNA II (+) or SignalSilence® GSK-3α siRNA I #6312 (+), using GSK-3α Antibody #9338 and β-Actin (13E5) Rabbit mAb #4970. GSK-3α Antibody confirms silencing of GSK-3α expression and β-Actin (13E5) Rabbit mAb is used to control for loading and specificity of GSK-3α siRNA. Western blot分析转染了100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-)， SignalSilence® GSK-3α siRNA II (+) 或者 SignalSilence® GSK-3α siRNA I #6312 (+)的HeLa细胞提取物，使用的抗体是GSK-3α Antibody #9338 和 β-Actin (13E5) Rabbit mAb 兔单抗#4970。GSK-3α Antibody 证实了GSK-3α表达的沉默，而β-Actin (13E5) Rabbit mAb兔单抗是作为GSK-3α siRNA上样和特异性的对照。 |
