| 货号 | 3982S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | GRK2 Antibody detects endogenous levels of total GRK2. This antibody may also detect GRK3, but does not cross-react with other GRKs. |
| 使用方法 | WB(1:1000) IP (1:25) |
| 供应商 | CST |
| 背景 | G-protein-coupled receptor kinase 2 (GRK2), also known as beta-adrenergic receptor kinase 1 (beta-ARK1), is a member of the GRK family, which phosphorylates the activated form of G-protein-coupled receptors (GPCRs) and initiates the desensitization process of GPCR (1). GRK2 kinase activity and cellular localization are tightly regulated by interactions with activated receptors, G-beta and G-gamma subunits, adaptor proteins, phospholipids, caveolin and calmodulin, as well as by phosphorylation (1). PKC phosphorylation enhances GRK2 activity by promoting its membrane localization and by abolishing the inhibitory association of calmodulin (2,3). PKA phosphorylates GRK2 at Ser685, which facilitates the association of GRK2 with a beta-adrenergic receptor (4). Erk inhibits GRK2 activity via phosphorylation at Ser670 (5). Src phosphorylates GRK2 at multiple tyrosine residues (Tyr13, 86 and 92), which activates GRK2 activity and promotes GRK2 degradation (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Western blot analysis of extracts from Ramos, THP1, U-937, and COS cells, using GRK2 Antibody. Western blot分析Ramos、THP1、U-937和COS细胞提取物，使用的抗体是GRK2 Antibody 兔多抗。 | |
Immunoprecipitation of GRK2 from Ramos, C6 and COS cell lysates followed by Western blot analysis, using GRK2 Antibody. Western blot分析后，使用GRK2 Antibody 兔多抗对Ramos、C6和COS细胞提取物进行GRK2的免疫共沉淀。 |
