| 货号 | 12794S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | GLDC Antibody recognizes endogenous levels of total GLDC protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Glycine decarboxylase (GLDC) is a component of a mitochondrial protein complex that catalyzes the degradation of glycine (1). The glycine cleavage system is composed of three distinct enzymes (P-, T- and L-proteins) and an additional component (H-protein) that transfers a glycine methylamine group from one enzyme to another. The GLDC protein (P-protein) is the decarboxylase that binds the methylamine group for transfer to the T-protein (2). Tumor-initiating cells in the primary non-small cell lung cancer (NSCLC) express high levels of GLDC and LIN28B, both of which are essential for the proliferation of tumor-initiating cells (3). GLDC is an oncogene that promotes tumorigenesis through its metabolic activity (3). Mutations in the corresponding GLDC gene account for the majority of reported cases of glycine encephalopathy, which is a metabolic disorder characterized by the accumulation of glycine, lethargy, hypotonia, intractable seizures, and death (4). |
| 存放说明 | -20C |
| 计算分子量 | 113 |
Western blot analysis of extracts from various tissues and cell lines using GLDC Antibody. 使用GLDC抗体对多种组织或细胞系提取物进行western blot分析。 | |
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human GLDC (hGLDC-Myc/DDK; +), using GLDC Antibody (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). 模拟转染(-)或转染了表达Myc/DDK标记full-length human GLDC (hGLDC-Myc/DDK; +)质粒的293细胞提取物，使用GLDC Antibody (上, Myc-Tag (71D10) Rabbit mAb 兔单抗#2278 (中), 或 GAPDH (D16H11) XP® Rabbit mAb 兔单抗#5174 (下)进行western blot分析。 |
