GKAP Antibody
货号：
13602S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 13602S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | GKAP Antibody recognizes endogenous levels of total GKAP protein. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Guanylate kinase-associated protein (GKAP, DLGAP1 or SAPAP1) is part of the postsynaptic scaffolding complex that includes the PSD-95, SAP90, and SHANK proteins (1-3). GKAP links the synaptic protein SHANK to a PSD-95 complex that includes NMDA glutamate receptors (3,4). Synaptic activity induces ubiquitination of GKAP protein by the E3 ubiquitin ligase TRIM3, which results in decreased GKAP protein levels through degradation (5,6). GKAP protein turnover is regulated by a CaMKII-dependent, bidirectional mechanism. Synaptic over-excitation leads to CaMKIIα-mediated GKAP phosphorylation at Ser346, which induces polyubiquitination of GKAP and removal of the scaffold protein from synapses. In contrast, during low-level synaptic activity CaMKIIβ phosphorylates GKAP, which triggers dissociation of GKAP from the motor protein complex responsible for GKAP transport to the base of the synapse and its subsequent incorporation into the postsynaptic density (7). |
| 存放说明 | -20C |
| 计算分子量 | 90-150 |
参考图片Western blot analysis of extracts from mouse brain, mouse lung, and rat brain using GKAP Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Western blot分析小鼠大脑，小鼠肺和大鼠大脑提取物，使用的抗体是GKAP Antibody 兔多抗(上) 或 β-Actin (D6A8) Rabbit mAb 兔单抗#8457 (下)。 |
