| 货号 | 4167S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | GGA3 Antibody detects endogenous levels of total GGA3 protein. GGA3 Antibody can also detect a carboxy-terminal GGA3 fragment derived from caspase-mediated cleavage. The antibody may also cross-react with other proteins of unknown origin. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | GGA3 is a member of the GGA family of proteins which also includes GGA1 and GGA2. These proteins consist of four distinct segments: a VHS domain that binds the di-leucine sorting signal DXXLL; a GAT domain that binds Arf-GTP; a hinge region that recruits clathrin; and a GAE domain that has sequence similarity to γ-adaptin and recruits a number of proteins. Arf1-GTPase recruits GGA3 to the trans-Golgi network. GGAs sort acid hydrolases to the lysosome and are involved in transporting proteins containing the DXXLL signal from the Golgi complex to the endosome (1). During apoptosis or cerebral ischemia, GGA3 is cleaved by caspase-3 at Asp313, reducing GGA3 levels and lysosomal degradation of β-secretase (BACE). The resulting elevated amount and activity of BACE plays a role in amyloid-β (Aβ) production, consistent with BACE elevation and Aβ accumulation in Alzheimer’s Disease (2). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Western blot analysis of extracts from mouse brain, Neuro2A and SH-SY5Y cells using GGA3 Antibody. Western blot分析小鼠大脑、Neuro2A和SH-SY5Y细胞提取物，使用的抗体是GGA3 Antibody 兔多抗。 |
