| 货号 | 5165S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The initiation of DNA replication in mammalian cells is a highly coordinated process that ensures duplication of the genome only once per cell division cycle. Origins of replication are dispersed throughout the genome and their activities are regulated via the sequential binding of pre-replication and replication factors. The origin recognition complex (ORC) is thought to be bound to chromatin throughout the cell cycle (1,2). The pre-replication complex (pre-RC) forms in late mitosis/early G1 phase beginning with the binding of CDT1 and cdc6 to the origin, which allows binding of the heterohexameric MCM2-7 complex. Once this complex is formed, the origin is “licensed” for initiation of DNA replication. In order to ensure that replication occurs only once per cell cycle, geminin binds to and inhibits CDT1 during the S, G2 and M phases. This prevents the recruitment of the MCM complex to the origins of replication, which blocks the premature reformation of the Pre-RC. At the metaphase/anaphase transition, geminin is degraded by the anaphase-promoting complex (APC) allowing for the formation of new pre-RC (3,4).哺乳动物细胞的DNA复制启动是一个高度协调的过程，从而保证了每次细胞分裂中基因组只复制一次。复制的起源分散在整个基因组中，其活性主要通过前复制因子和复制因子的连续结合来调控。起始识别复合体(ORC)被认为可以结合整个细胞周期的染色质(1,2)。前复制复合体(Pre-RC)在有丝分裂末期/G1早期随着CDT1和cdc6的结合而形成，允许异源六聚化的MCM2-7复合体的结合。复合体一旦形成，DNA复制起始被认可。为了保证每个细胞周期只发生一次DNA复制，联会蛋白结合并抑制S， G2和M期的CDT1。这阻止了MCM复合体被招募至复制起始物，从而阻遏了Pre-RC过早成熟的更新。在分裂中期/后期过渡期，联会蛋白被末期促进复合体(APC)降解从而促进新的pre-RC的形成(3,4)。 |
| 存放说明 | -20C |
| 计算分子量 | 25 |
Western blot analysis of extracts from various cell types using Geminin Antibody.Western blot 方法检测不同细胞提取物，使用的抗体为 Geminin Antibody。 | |
Immunoprecipitation of geminin from 293T cells using Geminin Antibody. Western blot was performed using the same antibody. Lane 1 contains 5% input.从293细胞中免疫沉淀geminin，使用Geminin Antibody。使用同一抗体进行western blot检测。泳道1为5% input。 |
