| 货号 | 3302S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | GCN2 Antibody detects endogenous levels of GCN2 protein independent of phosphorylation. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Phosphorylation of the alpha subunit of eukaryotic initiation factor 2 is a well documented mechanism of downregulating protein synthesis under a variety of stress conditions. Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) and hemin deficiency (HRI) can phosphorylate the alpha subunit of eIF2 (1,2). GCN2 is also required for UV-light induced translation inhibition, and in vivo phosphorylation of murine GCN2 at Thr898 is induced by both UV irradiation and by leucine deprivation (3). UV-induced activation of NF-kappaB also requires GCN2, which may act simply by preventing translation of IkappaB-alpha to replace pools that have been ubiquitinated and degraded (4). Interestingly, proteasome inhibitors (MG132 and ALLN) activate the GCN2/eIF2alpha pathway, suggesting a pivotal role for this kinase in stress response and ubiquitin-mediated signaling (5). In vitro autophosphorylation of yeast GCN2 within its activation loop at Thr882 and Thr887 (Thr898 and Thr903 in mouse) has also been reported (6). |
| 存放说明 | -20C |
| 计算分子量 | 220 |
Western blot analysis of extracts from ME180 and HT1376 cells that were untreated, treated with UV light (50mJ/cm2, 30 minutes), or subjected to nocodazole block (50 ng/ml, 24hrs), using GCN2 Antibody. 对ME180和HT1376细胞，分为未处理（-），或紫外线（50mJ/cm2，30分钟），或nocodazole抑制（50ng/ml，24小时）处理，使用GCN2 Antibody进行Western blot分析。 |
