| 货号 | 2263S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), that lacks the carboxy-terminal 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 38 FosB2. 48 FosB. |
Western blot analysis of extracts from HeLa cells serum-starved overnight and TPA-stimulated for 4 hours or NIH/3T3 cells serum-starved overnight and serum-stimulated for 4 hours, using FosB Antibody.Western blot 方法检测细胞提取物：无血清培养过夜的和TPA刺激4h的HeLa细胞，无血清培养过夜的和血清刺激4h的NIH/3T3细胞，使用的抗体是 FosB Antibody。 | |
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells starved overnight (left panels) and stimulated for 4 hours with TPA (right panels). Cells were stained with DAPI (upper panels) and analyzed by IF using FosB Antibody (lower panels).免疫荧光法检测：无血清培养的（左侧）、TPA处理4h后的（右侧）多聚甲醛固定HeLa 细胞，细胞用DAPI (上面) 染色，并用IF分析，使用的抗体是FosB Antibody (下面). |
