| 货号 | 4317S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | FMRP Antibody detects endogenous levels of total FMRP protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Fragile X syndrome, a frequent cause of inherited mental retardation, often results from expansion of the CGG trinucleotide repeat in the gene that encodes the fragile X mental retardation protein (FMRP) (1). FMRP (also known as FMR1) and its two autosomal homologs (FXR1 and FXR2) all bind RNA and play a role in the pathogenesis of fragile X syndrome (1-3). Each of these related proteins can associate with one another as well as form homodimers (3). FMRP can act as a translation regulator and is a component of RNAi effector complexes (RISC), suggesting a role in gene silencing (4). In Drosophila, dFMRP associates with Argonaute 2 (Ago2) and Dicer and coimmunoprecipitates with miRNA and siRNA. These results suggest that fragile X syndrome is related to abnormal translation caused by a defect in RNAi-related pathways (5). In addition, FMRP, FXR1, and FXR2 are components of stress granules (SG) and have been implicated in the translational regulation of mRNAs (6). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Western blot analysis of extracts from various cell lines using FMRP Antibody. 使用FMRP Antibody，免疫印迹（Western Blotting）分析不同细胞系提取物。 | |
Confocal immunofluorescent analysis of C2C12 cells, untreated or treated with MG-132 (10 μg/mL for 3 hours), using FMRP Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用FMRP Antibody (绿色)，共聚焦免疫荧光观察FMRP蛋白在C2C12细胞中定位，细胞分为MG-132 (10 μg/mL，3小时)处理组和未处理组， DY-554 phalloidin (红色)标记微丝蛋白。蓝色伪彩 = DRAQ5® #4084 (DNA 荧光染料)。 |
