| 货号 | 2877S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | FE65 Antibody detects endogenous levels of FE65. It does not cross-react with FE65L1 or FE65L2. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | FE65, FE65L1 and FE65L2 are members of the FE65 protein family. FE65 is an adaptor protein with protein-protein interaction domains including a WW domain followed by two phosphotyrosine interaction domains (PID1 and PID2) (1). Amyloid beta precursor protein (APP) binds to PID2 and undergoes sequential cleavage. First alpha-/beta secretases cleave and release the ectodomain into the extracellular environment. Subsequent processing by the gamma-secretase complex results in the APP intracellular domain (AICD) and the beta-amyloid peptides. The latter A-beta fragments form the main components of amyloid plaques in patients with Alzheimers disease (2). FE65 family members can regulate APP processing, resulting in elevated levels of A-beta (3). Double knock-out mice of FE65 and FE65L1 display a phenotype that occurs in animals lacking APP family members, supporting a functional interaction between FE65 and APP (4). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
Western blot analysis of extracts from mouse and rat brain, using FE65 Antibody. Western blot分析大鼠和小鼠大脑提取物，使用的抗体是FE65 Antibody 兔多抗。 | |
Western blot analysis of extracts from wild type (WT), FE65 knock-out (65KO), FE65L1 knock-out (L1KO) and FE65/FE65L1 double knock-out (DKO) mouse brain lysates, using FE65 Antibody. (Kindly provided by Dr. Suzanne Guénette, MassGeneral Institute for Neurodegenerative Disease, Charlestown, Massachusetts). Western blot分析野生型（WT）、FE65敲除（65KO）、FE65L1敲除（L1KO）和FE65/FE65L1双敲除（DKO）的小鼠大脑提取物，使用的抗体是FE65 Antibody 兔多抗。（由MassGeneral Institute for Neurodegenerative Disease, Charlestown, Massachusetts的Dr. Suzanne Guénette提供。） |
