| 货号 | 11910S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | EWS Antibody recognizes endogenous levels of total EWS protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The Ewing sarcoma (EWS) protein is a member of the multifunctional FET (FUS, EWS, and TAF15) family of proteins (1,2). These proteins are RNA and DNA binding proteins that are thought to be important for both transcriptional regulation and RNA processing. EWS can be found as part of a fusion protein with various E-twenty six (ETS) family transcription factors, most commonly Fli-1, in the Ewing sarcoma family of tumors (1-4). The amino terminus of the EWS protein, containing the transcriptional activation domain, is fused to the DNA binding domain of the ETS transcription factor, causing aberrant expression of target genes (1-5). EWS interacts with the transcription initiation complex via TFIID and RNA polymerase II subunits, as well as transcriptional regulators, such as Brn3A and CBP/p300, which suggests a role for EWS in transcriptional regulation (1,6-9). EWS also interacts with multiple components of the splicing machinery, implicating a role for EWS in RNA processing (1,10-12). EWS regulates the expression of cyclin D1, which controls G1-S phase transition during the cell cycle, at the level of transcriptional activation and mRNA splicing. The EWS-Fli-1 fusion protein has been shown to promote the expression of the cyclin D1b splice variant in Ewing sarcoma cells (13). In addition, EWS regulates the DNA damage-induced alternative splicing of genes involved in DNA repair and stress response and is required for cell viability upon DNA damage (14). Consistent with these results, EWS knockout mice display hypersensitivity to ionizing radiation and premature cellular senescence, suggesting a role for EWS in homologous recombination and maintenance of genomic stability (15). |
| 存放说明 | -20C |
| 计算分子量 | 85 |
Western blot analysis of extracts from various cell lines using EWS Antibody.Western blot方法检测不同细胞系的提取物，使用的抗体为EWS Antibody。 | |
Immunoprecipitation of EWS from HeLa cell extracts using Normal Rabbit IgG #2729 (lane 2) or EWS Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using EWS Antibody.从 HeLa细胞提取物中免疫沉淀EWS，使用的抗体为Normal Rabbit IgG #2729 (lane 2) 或 EWS Antibody (Mouse Specific) (lane 3)。Lane 1为10% input。 使用EWS Antibody进行Western blot检测。 |
