货号 | 13419S |
反应种属 | All |
来源宿主 | Rabbit |
应用 | W/IP |
使用方法 | WB(1:1000) IP (1:100) |
供应商 | CST |
背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged proteins biochemical properties. |
存放说明 | -20C |
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with amino-terminal or carboxy-terminal E-tag fusion protein (+), using E-Tag Antibody.Western blot检测293T细胞提取物,未转染(-)或转染了氨基端或羰基端E-tag融合蛋白(+),使用的抗体是E-Tag Antibody。 | |
Immunoprecipitation of transfected E-tag fusion protein from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or E-Tag Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using E-Tag Antibody.对293T细胞提取物中的E-tag融合蛋白进行免疫共沉淀,使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或E-Tag Antibody (泳道3)。泳道1为10%的上样。Western blot分析使用抗体是E-Tag Antibody。 |