| 货号 | 3885S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions such as those brought about by UV and certain chemicals occur (1). Excision Repair Cross Complementing 1 (ERCC1) forms a complex with XPF, which acts as the 5’ endonuclease required to excise the lesion (2). ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) (3) and involved in repair of double strand breaks (4). Expression of ERCC1 is related to survival rate and response to chemotherapeutic drugs in several human cancers including non-small cell lung cancer (5,6).DNA修复系统在所有的活细胞中运行管理各种DNA损伤。核苷酸切除修复(NER)在一些比较大的螺旋扭曲损伤的情况下被执行，这些损伤通常由紫外和化学物质引起(1)。切除修复交叉互补基因1(ERCC1)与XPF(充当损伤切除时的5’核酸内切酶)组成复合物(2)。ERCC1-XPF也参与DNA链间交联(ICLs) (3)修复和双链断裂修复(4)。在几种人类肿瘤中包括非小细胞肺癌发现ERCC1的表达和存活率相关并对化学治疗药物有反应(5,6)。 |
| 存放说明 | -20C |
| 计算分子量 | 39 |
Western blot analysis of extracts from HeLa and HepG2 cells using ERCC1 Antibody.Western blot方法检测HeLa和HepG2细胞提取物，使用的抗体为ERCC1 Antibody。 | |
Immunoprecipitation of ERCC1 from HeLa cells using ERCC1 Antibody. Western blot was performed using the same antibody. Lane 1 is 5% input.从HeLa细胞中免疫沉淀ERCC1，使用的抗体为ERCC1 Antibody。使用同一抗体进行western blot 检测。泳道1为5% input。 |
