| 货号 | 2428S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | EML4 Antibody detects endogenous levels of total EML4 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:25) |
| 供应商 | CST |
| 背景 | EML4 is a 120 kDa microtubule-associated WD-repeat protein of the EMAP (echinoderm microtubule-associated protein) family (1). The expression of EML4 is necessary for correct intracellular microtubule network formation (2). EML4 protein expression is upregulated during mitosis and downregulated during the remaining cell cycle (1). During mitosis, EML4 is heavily phosphorylated and associated with mitotic spindle (2). The amino-terminal segment of EML4 is essential for microtubule association and function. |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Western blot analysis of extracts from MCF7, A431, SHSY5Y and HepG2 cells, using EML4 Antibody 使用EML4 Antibody，免疫印迹（Western Blot）分析MCF7、A431、SHSY5Y和HepG2细胞中EML4蛋白水平。 | |
Confocal immunofluorescent analysis of SH-SY5Y cells using EML4 Antibody (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red). Blue psuedocolor = DRAQ5™ (flourescent DNA dye). 使用EML4 Antibody (绿色)和β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (红色)标记，共聚焦免疫荧光分析SH-SY5Y细胞。蓝色伪彩= DRAQ5™（DNA荧光染料）。 |
