| 货号 | 3263S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | eIF6 Antibody detects endogenous levels of total eIF6 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Eukaryotic initiation factor 6 (eIF6) is reqiured for the 60S ribosomal subunit assembly in the nucleolus (1). In the cytoplasm, this protein is bound to 60S ribosome subunits and prevents them from joining 40S ribosome subunits to form 80S ribosomes (2). eIF6 is also shown to associate with the RNA-induced silencing complex (RISC) (3). Deletion of eIF6 abolishes the miRNA-mediated gene silencing (3). eIF6 may play its essential role in miRNA-mediated silencing by inhibiting translation initiation or ribosome recycling (3). |
| 存放说明 | -20C |
| 计算分子量 | 27 |
Confocal immunofluorescent analysis of HeLa cells using eIF6 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用eIF6 Antibody (绿色)，共聚焦免疫荧光观察eIF6蛋白在HeLa细胞中定位。DY-554 phalloidin (红色)标记微丝蛋白。蓝色伪彩 = DRAQ5® #4084 (DNA 荧光染料)。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using eIF6 Antibody in the presence of control peptide (left) or antigen-specific peptide (right). 使用eIF6 Antibody，免疫组化分析人类结肠癌组织石蜡切片，其分别孵育对照多肽 (左图)和抗原特异性多肽(右图)。 | |
Western blot analysis of extracts from various cell types using eIF6 Antibody. 使用eIF6 Antibody，免疫印迹（Western Blotting）分析不同细胞系的提取物。 |
