| 货号 | 2444S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | eIF4H Antibody detects endogenous levels of total eIF4H protein (both long and short isoforms). |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | A variety of factors contribute to the initiation of translation. Eukaryotic translation initiation factor 4H (eIF4H) was purified to near homogeneity from rabbit reticulocyte lysate and shown to stimulate translation in an assay deficient in eIF4F and eIF4B (1). eIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors eIF4A and eIF4B (1,2). eIF4H was further shown to stimulate the initial rate and extent of eIF4A-mediated mRNA secondary structure unwinding (3). Interaction between eIF4H and the herpes simplex virus shutoff protein (Vhs) appears to be important for Vhs-mediated degradation of mRNA (4). Deletion of a large region of chromosome 7, including the corresponding eIF4H gene, results in Williams-Beuren Syndrome (WBS), an autosomal dominant disorder that can present with cardiovascular problems, mental retardation and distinctive facial features (5). |
| 存放说明 | -20C |
| 计算分子量 | 25, 27 |
Confocal immunofluorescent analysis of PANC-1 cells using eIF4H Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用eIF4H Antibody (绿色)，共聚焦免疫荧光观察eIF4H蛋白在PANC-1细胞中定位。Alexa Fluor® 555 phalloidin (红色)标记微丝蛋白。蓝色伪彩 = DRAQ5® #4084 (DNA 荧光染料)。 | |
Western blot analysis of extracts from various cell types using eIF4H Antibody. 使用eIF4H Antibody，Western Blotting分析不同细胞系中eIF4H蛋白水平。 |
