| 货号 | 3692S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | eEF2k Antibody detects endogenous levels of total eEF2k independent of phosphorylation. |
| 使用方法 | WB(1:1000) IP (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Eukaryotic elongation factor 2 kinase (eEF2k) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation (1). eEF2k is normally dependent on Ca2+ ions and calmodulin (2,3). It can be activated by PKA in response to elevated cAMP levels (4-6), which are generally increased in stress- or starvation-related conditions. eEF2k can also be regulated in response to a wide range of stimuli that promote cell growth and protein synthesis. This involves the phosphorylation of eEF2k by p90RSK and p70 S6 kinase at Ser366 or by SAPK4/p38delta at Ser359, leading to the inactivation of eEF2k (7,8), which facilitates the dephosphorylation of eEF2, and thus promotes translation. |
| 存放说明 | -20C |
| 计算分子量 | 105 |
Western blot analysis of extracts from C6, 293, HeLa and SW-13 cells using eEF2k Antibody. 使用eEF2k Antibody，免疫印迹（Western Blotting）分析C6, 293, HeLa和SW-13细胞提取物。 | |
Confocal immunofluorescent analysis of HeLa cells using eEF2k Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用eEF2k Antibody (绿色)，共聚焦免疫荧光观察eEF2k蛋白在HeLa细胞定位。 DY-554 phalloidin (红色)标记微丝蛋白。蓝色伪彩 = DRAQ5® #4084 (DNA 荧光染料)。 |
