| 货号 | 2332S |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | eEF2 Antibody detects endogenous levels of total eEF2 independent of phosphorylation. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. It has been shown that phosphorylation of eEF2 at threonine 56 by eEF2 kinase inhibits its activity (1-4). eEF2 kinase is normally dependent on Ca2+ ions and calmodulin (5,6). eEF2 kinase can also be activated by PKA in response to elevated cAMP levels (7-9), which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation. The inactive phosphorylated eEF2 can be converted to its active nonphosphorylated form by a protein phosphatase, most likely a form of protein phosphatase-2A (PP-2A). Insulin, which activates protein synthesis in a wide range of cell types, induces rapid dephosphorylation of eEF2 through mTOR signaling and may involve modulation of the activity of the PP-2A or the eEF2 kinase or both (10). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
Western blot analysis of extracts from C6 cells, untreated or forskolin-treated (10 µM for 60 minutes), using Phospho-eEF2 (Thr56) Antibody (upper) or eEF2 Antibody #2332 (lower). 使用Phospho-eEF2 (Thr56) Antibody (上图)和eEF2 Antibody #2332 (下图)，免疫印迹（Western Blotting）分析C6细胞的提取物。 | |
Confocal immunofluorescent analysis of HeLa cells using eEF2 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用eEF2 Antibody(绿色)，共聚焦免疫荧光观察eEF2蛋白在HeLa细胞定位。 DY-554 phalloidin (红色)标记微丝蛋白。蓝色伪彩 = DRAQ5® #4084 (DNA 荧光染料)。 |
