| 货号 | 3838S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-F |
| 目标/特异性 | EAAT2 Antibody detects endogenous levels of total EAAT2 protein. |
| 使用方法 | WB(1:1000) IF-F (1:100) |
| 供应商 | CST |
| 背景 | Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. During neurotransmission, glutamate is released from vesicles of the pre-synaptic cell, and glutamate receptors (e.g. NMDA Receptor, AMPA Receptor) bind glutamate for activation at the opposing post-synaptic cell. Excitatory amino acid transporters (EAATs) regulate and maintain extracellular glutamate concentrations below excitotoxic levels. In addition, glutamate transporters may limit the duration of synaptic excitation by an electrogenic process in which the transmitter is cotransported with three sodium ions and one proton, followed by countertransport of a potassium ion. Five EAATs (EAAT1-5) are characterized: EAAT2 (GLT-1) is primarily expressed in astrocytes but is also expressed in neurons of the retina and during fetal development (1). Homozygous EAAT2 knockout mice have spontaneous, lethal seizures and an increased predisposition to acute cortical injury (2). PKC phosphorylates Ser113 of EAAT2 and coincides with glutamate transport (3). |
| 存放说明 | -20C |
| 计算分子量 | 65 |
Confocal immunofluorescent analysis of rat retina using EAAT2 Antibody (green). Blue pseudocolor = DRAQ5® #4084(fluorescent DNA dye). 共聚焦免疫荧光分析大鼠视网膜细胞，使用EAAT2 Antibody（绿色）进行标记。蓝色伪彩=DRAQ5® #4084(荧光DNA染料)。 | |
Western blot analysis of extracts from mouse and rat brain using EAAT2 Antibody. Western blot分析小鼠和大鼠大脑提取物，使用的抗体是EAAT2 Antibody 兔多抗。 |
