| 货号 | 3742S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/ChIP |
| 使用方法 | WB(1:1000) ChIP (1:100) |
| 供应商 | CST |
| 背景 | The E2F transcription factors are essential for regulation of the cell cycle (1,2). Physiological E2F is a heterodimer composed of an E2F subunit together with a DP subunit (3, 4). Six members of the E2F family have been identified, and each E2F subunit has a DNA binding and a dimerization domain. E2F-1 to -5 activate transcription. E2F-1 to -3 bind pRb, and E2F-4 and -5 bind p107 or p130, and these interactions are under cell cycle control (5-8). E2F-1 has oncogenic properties in vivo and in vitro. E2F-1 can induce apoptosis through p53-dependent and -independent mechanisms. E2F-1 is stress-responsive, and is regulated by a PI3-kinase-like kinase family such as the ATM/ATR kinases (9-11).E2F转录因子对于细胞周期的调节必不可少(1,2)。生理性的E2F是由一个E2F亚基伴随一个DP亚基组成的异源二聚体(3, 4)。E2F家族的六个成员已被鉴定，每一个E2F亚基都有一个DNA结合和二聚化结构域。E2F 1 到5能够激活转录。E2F 1到3能够结合pRb，而E2F-4和E2F-5能够结合p107或者p130，这些相互作用都接受细胞周期的控制(5-8)。E2F-1离体或在体均具有致癌基因的特性。E2F-1能够通过p53依赖或者非依赖的机制诱导凋亡。E2F-1是应激反应性的，并可以被PI3激酶样激酶家族如ATM/ATR激酶调控(9-11)。 |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Western blot analysis of extracts from HeLa cells, untransfected (-) or transfected with E2F1 siRNA (+) to silence E2F1 protein expression, using E2F-1 Antibody.Western blot方法检测未转染的（-）或转染siRNA（+）以沉默E2F1蛋白表达的HeLa细胞提取物，使用的抗体为E2F-1 Antibody。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Raji cells and either 5 μl of E2F-1 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using using SimpleChIP® Human Timeless Intron 1 Primers #7001, human DHFR promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫沉淀方法检测4x106 Raji细胞交联染色质，分别使用5μl E2F-1 Antibody或者2 μl Normal Rabbit IgG #2729，使用的试剂盒为 SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。富集的DNA用real-time PCR技术定量，使用的引物为SimpleChIP® Human Timeless Intron 1 Primers #7001，human DHFR promoter primers,，以及SimpleChIP® Human α Satellite Repeat Primers #4486。以input染色质总量为1，每个样品中IP的DNA量用相对于input 染色质的相对数表示。 | |
Western blot analysis of extracts from HeLa cells, untransfected (-) or transfected with E2F1 siRNA (+) to silence E2F1 protein expression, using E2F-1 Antibody.Western blot方法检测未转染的（-）或转染siRNA（+）以沉默E2F1蛋白表达的HeLa细胞提取物，使用的抗体为E2F-1 Antibody。 |
