| 货号 | 2703S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | DYRK1B Antibody detects endogenous levels of total DYRK1B protein. This antibody detects the three major alternative splicing variants reported for DYRK1B. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The DYRK family includes several dual-specificity tyrosine-phosphorylated and regulated kinases capable of phosphorylating proteins at both tyrosine and serine/threonine residues (1). The DYRK family was identified based on homology to the yeast Yak1 (2) and the Drosophila minibrain (mnb) kinases (3). Seven mammalian isoforms have been discovered, including DYRK1A, DYRK1B, DYRK1C, DYRK2, DYRK3, DYRK4, and DYRK4B. Differences in substrate specificity, expression, and subcellular localization are seen across the DYRK family (4,5). All DYRK proteins have a Tyr-X-Tyr motif in the catalytic domain activation loop, with phosphorylation of the second Tyr residue (e.g. Tyr312 of DYRK1A) necessary for kinase activity. DYRKs typically autophosphorylate the tyrosine residue within their activation loop, but phosphorylate substrates at serine and threonine residues (1,6).In contrast to the ubiquitous DYRK1A, DYRK1B exhibits relatively restricted expression with highest levels found in the testis and muscle (7,8). Three major DYRK1B splice variants demonstrate distinct expression patterns and functional properties (9). DYRK1B plays a critical role in myoblast differentiation by affecting cell motility, transcription, cell cycle progression and survival (10,11). In addition, DYRK1B contributes to the survival of certain cancer cells (7,12,13). |
| 存放说明 | -20C |
| 计算分子量 | 70-80 |
Western blot analysis of extracts from various cell lines and rat testes using DYRK1B Antibody. Western blot分析多个细胞系和大鼠睾丸提取物，使用的抗体是DYRK1B Antibody 兔多抗。 |
