| 货号 | 3605S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | DIDO1 Antibody detects endogenous levels of DIDO1 and other isoforms including DIDO2 and DIDO3. An unknown band is detected in HepG2 cells at 35 kDa. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | The putative transcription factor DIDO1 (death inducer obliterator 1, also termed DIO-1 or DATF1) contains a pair of zinc finger motifs and is upregulated by apoptotic stimuli. DIDO1 is expressed in the developing limb and may play a role in controlling programmed cell death during development (1-3). Nuclear translocation of DIDO1 during apoptosis is associated with its apoptotic activity (2). Alternative splicing produces the DIDO-1, -2 and -3 isoforms (also termed DIO-1, -2, -3), whose targeted disruption in mice produces a phenotype similar to myelodysplastic/myeloproliferative disease (MPS/MPD) in humans (3). DIDO3, the largest of the splice variants, is associated with the centrosome and plays a role in mitotic checkpoint and chromosome stability (4). |
| 存放说明 | -20C |
| 计算分子量 | 70, 80, 130, 247 |
Western blot analysis of extracts from COS cells, mock transfected or transfected with a construct overexpressing human DIDO1, using DIDO1 Antibody. Western blot方法检测COS细胞提取物，空质粒（-）或过表达人DIDO1载体转染；使用抗体为DIDO1 Antibody。 | |
Western blot analysis of extracts from K-562 and HepG2 cells, untreated or treated with Staurosporine #9953 (1 μM for 4 hours), using DIDO1 Antibody. Western blot 检测 K-562和HepG2 细胞提取物，未处理 (-)或Staurosporine #9953 (1 μM，4hr)处理，使用抗体为DIDO1 Antibody。 |
