| 货号 | 9732S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | DFF45/DFF35 Antibody detects endogenous levels of total full length DFF45 and/or full length DFF35. The antibody does not cross-react with related proteins. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Human DFF45 and its mouse homologue ICAD function in normal cells as chaperones for caspase-activated deoxyribonuclease (DFF40 or CAD) during its synthesis (1). The association of DFF45 (or its isoform DFF35) with DFF40 inhibits the DNAse activity of the latter (1-4). In vitro, DFF45 has been shown to be the target of several caspases, including caspase-3, -6, -7, -8 and granzyme B (3). In vivo, caspase-3 is believed to be the primary enzyme responsible for processing DFF45 and release of its carboxy-terminal fragment (3,5). The cleavage of DFF45 inactivates its inhibitory function on DFF40 and causes nuclear DNA degradation by DFF40, leading to cell death (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 35, 45 |
Western blot analysis of Jurkat cells, untreated (control) or etoposide-treated (25 µM), using DFF45/DFF35 Antibody. 采用抗体DFF45/DFF35 Antibody对未处理（对照）或 etoposide (依托泊苷)(25 µM)处理的Jurkat细胞进行Western blot分析 |
