Dab1 Antibody
货号：
3328S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 3328S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Dab1 Antibody detects transfected levels of Dab1 protein. This antibody does not cross-react with other proteins. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The Reelin signaling pathway plays a critical role in neuronal development. Reelin is a secreted glycoprotein that binds to the lipoprotein receptors VLDLR and ApoER2 or alpha3beta1 integrin on the surface of neurons (1,2). Activation of these receptors induces tyrosine phosphorylation of Disabled 1 (Dab1), an intracellular adaptor. It is generally believed that tyrosine phosphorylation of Dab1 by Src family tyrosine kinases is the most critical downstream event in Reelin signaling. The phosphotyrosine-binding (PTB) domain within its amino terminus enables Dab1 to recognize and bind to a conserved sequence motif within the cytoplasmic tail of the receptors. In addition, the PTB contains a Pleckstrin Homology-like subdomain that binds to phosphoinositides. The phosphoinositide-binding region within the Dab1 PTB domain is required for membrane localization and basal tyrosine phosphorylation of Dab1 independent of VLDLR and ApoER2 (3). It has been demonstrated that Src, CrkII, CrkL and Dock1 associate with tyrosine-phosphorylated Dab. The CrkII-Dab1 interaction requires tyrosine phosphorylation of Dab1 at residues 220 or 232 (4). |
| 存放说明 | -20C |
| 计算分子量 | 80, 110 GFP-Dab1 fusion. |
参考图片Western blot analysis of cell extracts from 293 cells (lane 1) and 293 cells transfected with a GFP-Dab1 expression construct (lane 2), using Dab1 Antibody (upper) and GFP antibody (lower). Western blot分析未处理的293细胞（列1），和转染GFP-Dab1表达载体的293细胞（列2）提取物，使用的抗体是Dab1 Antibody 兔多抗（上图）或GFPAntibody 兔多抗。 |
