| 货号 | 13086S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | CTR1/SLC31A1 Antibody recognizes endogenous levels of total CTR1 (SLC31A1) protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The high affinity copper uptake protein 1 (CTR1, SLC31A1) helps maintain copper homeostasis by mediating dietary copper intake chiefly in the small intestine (1). A series of methionine-rich repeats and other residues are conserved among CTR1 genes across taxa, and are thought to be important for copper transport (2,3). In mammalian cells, CTR1 is localized to the plasma membrane and intracellular vesicles (3). Upon copper uptake via plasma membrane into cells, CTR1 is down regulated by clathrin-dependent endocytosis and degradation of CTR1 protein (4). Research studies suggest that the CTR1 copper transporter also mediates uptake of the anticancer drug cisplatin in yeast and mammals and that decreased CTR1 can result in the development of cisplatin resistance (5,6). Treatment of cancer cells with cisplatin can result in reduced CTR1 expression, which reduces cisplatin accumulation within cells and leads to cisplatin resistance in some human cancer cells (7-9). |
| 存放说明 | -20C |
| 计算分子量 | 26-34 |
Western blot analysis of extracts from Caco-2 and SK-MEL-3 cells using CTR1/SLC31A1 Antibody. 使用CTR1/SLC31A1 Antibody对Caco-2 和SK-MEL-3细胞提取物进行western blot分析。 | |
Immunoprecipitation of CTR1 from Hep G2 cell extracts using Normal Rabbit IgG #2729 (lane 2) or CTR1/SLC31A1 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CTR1/SLC31A1 Antibody.使用Normal Rabbit IgG #2729 (lane 2) 或 CTR1/SLC31A1 Antibody (lane 3)对Hep G2细胞提取物中的CTR1进行免疫沉淀实验。Lane1是10%上样量。使用CTR1/SLC31A1 Antibody进行western blot分析。 |
