| 货号 | 13256S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | CtBP2 Antibody recognizes endogenous levels of total CtBP2 protein. This antibody does not cross-react with the CtBP1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | CtBP2 (carboxy-terminal binding protein-2) and its homolog CtBP1 are transcriptional co-repressors originally identified as proteins that bind the carboxy-terminus of the human adenovirus E1A protein (1-3). CtBP proteins are thought to play important roles in regulating various developmental pathways because deletion of CtBP2 leads to embryonic lethality at E10.5 and is correlated with axial patterning defects (4). CtBP proteins regulate various oncogenic signaling pathways as promoters of epithelial-mesenchymal transition, apoptosis antagonists, and tumor suppressor genes repressors (1,5). The CtBP protein transcription co-repression activity results from interactions with numerous transcription factors and chromatin modulators, including the polycomb group proteins (1,6,7). Depending on the context, CtBP proteins interact with a short amino acid sequence motif (PXDLS) to mediate repression of target genes through both histone deacetylase-dependent and independent mechanisms (6,8,9). CtBP proteins display a high sequence homology to the bacterial D-isomer-specific 2-hydroxyacid dehydrogenase enzymes. Research studies indicate that nuclear NADH levels regulate CtBP transcription repression activities, as NADH binding is required for CtBP2 homodimerization and transcription co-repressor activity (6,9-11). |
| 存放说明 | -20C |
| 计算分子量 | 47 |
Western blot analysis of extracts from various cell lines using CtBP2 Antibody. 使用CtBP2抗体对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of CtBP2 from HeLa cell extracts using Normal Rabbit IgG #2729 (lane 2) or CtBP2 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CtBP2 Antibody. 使用Normal Rabbit IgG #2729 (lane 2) 或 CtBP2 抗体 (lane 3)对HeLa细胞提取物中的CtBP2蛋白进行免疫沉淀实验。Lane1 是10%上样量。使用CtBP2抗体进行western blot分析。 |
